FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12571625
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG943090.750173505811699TruSeq Adapter, Index 13 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG99650.037.321211
ATGCCGG33000.036.9079470
CGTGTGC172600.031.3064381
GTGTGCT196650.027.5602762
GACGTGT48000.025.5349671
AAAAAGG251750.022.38261870
CTGCTTG335450.022.21284158
TGCTTGA351100.021.43205859
GCTTGAA362250.021.1492760
TGTGCTC259050.020.9884623
GTGCTCT266850.020.388094
CTTGAAA403050.019.1820461
TCTGCTT400350.018.7430957
CTTCTGC414950.018.6066355
CCGTCTT419300.017.87082551
TTCTGCT434750.017.64644456
GTCTTCT430050.017.43224353
GCCGTCT435200.017.20994250
TCTTCTG441000.017.04708954
TGCCGTC448200.016.9528449
Produced by FastQC (version 0.11.2)