FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_dna2-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_dna2-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17239687
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG699610.40581363223125805TruSeq Adapter, Index 16 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT61600.028.5380081
CTGCTTG251400.024.79425658
ACGTGTG92700.023.799151
TGCTTGA277300.022.44065759
GCTTGAA311950.020.82322360
ATGCCGG43500.020.59696270
CCGTCTT297450.020.55571651
CGTGTGC122900.020.4299771
TGCCGTC317550.020.38987549
GCCGTCT302650.019.9596950
ATGCCGT336100.019.41024848
TATGCCG349350.018.89452247
TCTGCTT338450.018.68598757
CGTCTTC332050.018.38216652
CTTCTGC354550.017.96584155
GTGTGCT146900.017.279252
GTATGCC386700.017.16912346
CTTGAAA394150.016.81796361
GTCTTCT364100.016.5622153
CATCTCG331550.016.42533540
Produced by FastQC (version 0.11.2)