FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_dna2-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_dna2-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482447
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG4615103.423043309571326TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG264540.19621067303287007TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAGCTCGTATG233490.1731807289878462TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGTATG154850.11485303817623017TruSeq Adapter, Index 7 (97% over 36bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG136870.10151718007866078TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG656500.046.90908470
GAAAAAA3248700.045.8339370
TGAAAAA3220550.045.77886669
TTGAAAA3059050.045.42091468
CTTGAAA3041600.045.3571867
GCTTGAA3081750.044.2847266
ACGTGTG626450.043.819381
TGCTTGA3041050.043.6484365
CTGCTTG3057400.043.21697664
TTCTGCT3060800.042.6269762
CTTCTGC3176400.042.57744261
TCTGCTT3137700.042.4255263
CCCCCAG38150.042.3964842
TCTTCTG3194300.041.46481360
GTCTTCT3195600.041.44450459
CCCCTAG17050.041.2718662
AAAAAAG787900.039.8763469
GCGTGAA142550.038.98852566
CCCCAGA85500.038.8574643
GACGTGT221100.038.8350331
Produced by FastQC (version 0.11.2)