FastQCFastQC Report
Thu 31 May 2018
H2HLVAFXY_n01_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HLVAFXY_n01_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171521
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG428060.32498904264739054TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG52750.029.5430771
CGTGTGC77700.026.2763921
ATGCCGG22200.024.27851370
CTGCTTG147900.021.4868158
GTGTGCT96500.021.2241172
GCGCGGG33600.021.14516470
CGCGCGG36850.019.37516269
TGCTTGA167300.019.22555459
GACGTGT31850.018.8020361
GCTTGAA190650.017.43991960
CCGTCTT184250.017.36153451
TGCCGTC190500.017.14099749
CCCCGGG43200.017.0136767
TGTGCTC123300.016.7246323
GCCGTCT191400.016.62154250
ACGCGCG42950.016.29744568
CCCGGGT45550.016.2895878
TGCCCCG44800.016.2551635
GTGCTCT128650.016.1108324
ATGCCGT205850.015.77780248
Produced by FastQC (version 0.11.2)