FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21054152
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1395210.6626768914748977No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA825120.39190369671502323TruSeq Adapter, Index 22 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG103750.063.0458648
ATGCCGT116100.056.1908649
GTATGCC120500.054.48198347
CGTATGC136900.048.20763446
TGCCGTC142250.046.10407350
TCGTATG166300.039.93361745
CTCGTAT163850.039.68853844
GCCGTCT165250.039.604651
TACGTAA191700.033.7966536
TCTCGTA203550.031.96470843
GTAATCT204800.031.43233939
GTACGTA216350.029.94600135
TAATCTC219000.029.75692240
ACGTAAT224150.029.08862737
CCGTCTT230550.028.53687152
CCCGGTT54100.028.066061
CGTAATC229850.028.00671238
CGTACGT229850.027.99203734
CGCGCCT20100.027.98451
TGAAAAA250450.026.7777764
Produced by FastQC (version 0.11.2)