FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209203
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3823272.0996361015910474TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1526660.8384002309162021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG455700.067.0384948
ATGCCGT464300.065.7373249
GTATGCC470700.064.9525547
CGTATGC482850.063.32546646
TGCCGTC483350.063.07525350
GCCGTCT503700.060.3082651
TCGTATG508700.059.9507345
CTCGTAT496800.059.62986444
TCTCGTA528400.055.8026543
AGGGGGG113800.055.3402861
CCGTCTT555350.054.8487352
CGGAATC568800.051.41432638
CTTGAAA593050.051.3485562
CTGCTTG594350.050.7775459
TGAAAAA612250.050.27343464
CTTCTGC608900.049.87730856
TTCTGCT609050.049.7061457
TCTGCTT611600.049.57127458
GGAATCT589850.049.4098939
TCGGAAT614150.049.1178837
Produced by FastQC (version 0.11.2)