FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14700892
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA273585418.610122433387037TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1332820.906625257841497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA178950.121727307431413TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3133750.068.0313548
ATGCCGT3137800.067.8973749
GTATGCC3142750.067.8792847
GGCCTTA3106950.067.7442736
TATCTCG3074350.067.7413141
CGTATGC3153800.067.7386446
TGCCGTC3148300.067.6185550
CTTATCT3060200.067.5688739
GCCTTAT3095900.067.5492537
TTATCTC3074250.067.4910340
GTGGCCT3126300.067.4309134
GCCGTCT3152550.067.41299451
TCGTATG3162650.067.4122745
CGTGGCC3144750.067.4105233
CTCGTAT3128550.067.40050544
TCTCGTA3122100.067.0754343
GTCACGT3195750.066.9774229
TCACGTG3191200.066.9659330
AGTCACG3212800.066.79301528
ACGTGGC3186900.066.77019532