FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340529
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8645105.290587593584027TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1528030.9351166048541023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1061250.067.42419448
ATGCCGT1070650.066.7516849
CGTATGC1076750.066.6460546
GTATGCC1077850.066.4784147
TGCCGTC1089450.065.52061550
GCCGTCT1100500.064.79201551
TCGTATG1105250.064.71818545
CTCGTAT1088800.064.2638144
CGTGAAA1111950.064.0274933
ACGTGAA1116400.063.95693632
CACGTGA1116350.063.90823731
AGTCACG1126000.063.70205728
GTGAAAC1102900.063.5169134
TCTCGTA1092800.063.18548643
TGAAACG1109750.063.1248535
GTCACGT1138750.062.8486729
TCACGTG1140950.062.67829530
CAGTCAC1158700.062.32493627
CCGTCTT1149950.061.99383552
AAACGAT1099600.061.6870637
Produced by FastQC (version 0.11.2)