FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21327867
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2761561.2948130256063581TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1195840.5606936689918406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG338450.065.7776748
ATGCCGT354450.062.78897549
GTATGCC360150.061.9577947
CGTATGC364450.061.22706246
CCGCACA354150.060.4539536
CGCACAT364900.058.30425337
TGCCGTC382100.058.29091650
GCCGTCT404100.055.0333651
TCGTATG411550.054.22701345
TCCGCAC400050.054.19898635
CTCGTAT410250.053.53258544
CACATCT395450.053.27609639
GTCCGCA408550.053.24868834
CGTCCGC416800.052.31909633
GCACATC412650.051.16413538
TCTCGTA425300.051.0947643
AGTCACG446000.050.2532628
GTCACGT447650.049.9593129
TCACGTC453400.048.86639430
CATCTCG437200.048.60709441
Produced by FastQC (version 0.11.2)