FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17810352
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2923121.641247741762768TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG952520.5348125629409234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG368950.066.48276548
ATGCCGT382800.064.0683549
CGTATGC390200.063.127446
GTATGCC390050.063.0276847
CGTCCCG380250.062.88235
CCGTCCC380950.062.84795434
CCCGATC364950.061.5760238
CCCGTCC391300.061.50368533
GTCCCGA384200.061.16696536
CACCCGT393150.061.14768231
TCACCCG398450.060.44688830
TGCCGTC408300.060.16892650
GTCACCC404350.059.6189329
ACCCGTC406350.059.17699432
GCCGTCT424000.057.7217751
CTCGTAT417200.057.13099344
TCGTATG429450.057.12404645
AGTCACC428700.056.7161228
TCCCGAT424650.054.25189637
TCTCGTA432900.053.93521543