FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27328050
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA665111824.33806290606172TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2168290.793430193519113No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA760340.27822694996532865TruSeq Adapter, Index 15 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA533570.195246276261936TruSeq Adapter, Index 15 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG513550.18792046999328527TruSeq Adapter, Index 15 (97% over 37bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA353390.12931401984407961TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA302800.11080190500236936TruSeq Adapter, Index 15 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA294790.10787085064613097TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA290180.1061839392126405TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA278520.10191726083639337TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7885850.067.01649548
ATGCCGT7887050.066.9153249
GTATGCC7909450.066.85367647
CGTATGC7920700.066.8293346
TGCCGTC7905850.066.6655550
CTCGTAT7790100.066.5474744
GCCGTCT7905100.066.5133451
TCGTATG7946400.066.5096945
ATGTCAG7944300.066.4688434
TCACATG7992500.066.4000330
CACATGT7988750.066.3828331
ACATGTC7981650.066.3747832
TGTCAGA7928450.066.35892535
TCTCGTA7787200.066.3404443
GTCACAT8016800.066.2972629
CATGTCA7989350.066.2284433
GTCAGAA7927550.066.1706336
AGTCACA8060600.066.1016128
CCGTCTT7954800.066.0734252
TCAGAAT7926400.065.9787237
Produced by FastQC (version 0.11.2)