FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22181972
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6927523.1230406385870473TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1938410.8738673008874053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG817650.066.8410848
ATGCCGT833850.065.5673249
CGTATGC838400.065.2827746
GTATGCC840400.065.0643647
TGCCGTC863750.063.1869250
TATCTCG843350.063.0988141
CGTATCT842550.062.6001739
TCGTATG884100.061.88426245
GCCGTCT883700.061.6184951
CTCGTAT879700.061.4369144
GTATCTC871350.060.8653140
CCGTATC867100.060.83176438
TCTCGTA900400.059.641243
TCCGTAT906850.058.8706837
TCACAGT942450.057.98208230
AGGGGGG174450.057.8413621
GTCACAG947400.057.72674629
CCGTCTT943400.057.70466652
CACAGTT953300.057.2968331
AGTCACA976650.056.39404728
Produced by FastQC (version 0.11.2)