FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17857914
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4353372.437781926825272TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1094970.6131567214401413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG522700.065.8764248
ATGCCGT536250.064.2440249
CGTATGC542100.063.6332946
GTATGCC542550.063.57415447
TGCCGTC557850.061.80029750
TCGTATG576150.059.8298645
GCCGTCT577300.059.5993151
CTCGTAT573200.058.8616744
AGTCAAC583100.058.84442534
CACAGTC590800.058.7758331
GTCACAG594200.058.6290929
TCACAGT599600.058.10900530
ACAGTCA603450.057.54863432
TCTCGTA588350.057.23457743
AGTCACA614000.057.02005428
CAGTCAA604050.057.01534333
GTCAACA603550.056.87919235
ACAATCT599850.055.64798739
CAGTCAC636350.055.1375927
AACAATC608900.054.91721738
Produced by FastQC (version 0.11.2)