FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22549036
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1507110.668370035863174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA875100.38808754396418543TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG110800.062.55228848
ATGCCGT119600.057.97862249
GTATGCC136500.051.7603847
TATCTCG142600.047.8042641
TGCCGTC147550.047.25370850
CGTATGC147900.047.23487546
CCTTTAT142400.047.144537
GCCGTCT164950.042.3541551
TTTATCT162650.041.52895739
TTATCTC169150.040.19886440
TCGTATG189050.037.19012545
CTTTATC183100.036.87184538
TCCTTTA188200.036.12977236
CGCGCCT25550.031.334961
GTCACAT230950.030.2676329
CTCGTAT234550.029.99037644
CCGTCTT234400.029.96734252
CCCGGTT64550.029.08271
ACATTCC243250.028.50810632
TGAAAAA251050.028.37638764
Produced by FastQC (version 0.11.2)