FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19897915
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8671024.357753061061925TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311160.6589434119102429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG989650.066.9731348
ATGCCGT998600.066.3625249
TATATCT1000750.065.6905239
GTATGCC1012150.065.62665647
CGTATGC1014600.065.4139546
TATCTCG1006250.065.3144941
TGCCGTC1015100.065.2056550
GATATAT1024650.064.8250837
ATATATC1024500.064.3629838
TGATATA1034900.064.2230336
GCCGTCT1034700.063.8715851
ACTGATA1044450.063.67874534
CTGATAT1047650.063.41179735
CACACTG1056900.062.98217831
TCGTATG1053250.062.96998245
ACACTGA1059350.062.88783332
ATATCTC1057450.062.27603540
GTCACAC1076650.061.98180829
CACTGAT1078400.061.7066333
TGAAAAA1100850.060.51014364
Produced by FastQC (version 0.11.2)