FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_tmd10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_tmd10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18713800
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1749770.9350158706409173No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1270110.6787023479998717TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG158400.063.16045848
ATGCCGT165700.060.39870549
GTATGCC179150.056.3645547
CGTATGC187200.053.62803646
TGCCGTC188500.053.09361650
AGGGGGG92400.052.80811
TATCTCG181800.052.56385441
GCCGTCT207400.048.2400751
TCGTATG221950.045.35577445
ATATCTC227550.042.23821340
TGGATAT247550.039.31347737
CTCGTAT254650.039.1522444
CCGTCTT262900.038.20061552
GTGGATA256800.037.8167936
GGATATC258200.037.46426838
GATATCT258700.037.21850239
CTTGAAA277450.036.3202962
TGAAAAA287950.035.24738364
CTGCTTG303650.033.04966759
TCTCGTA304350.032.65670843