FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11486790
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1883201.6394484446916848TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG872970.7599773304813616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG243600.065.13158446
ATGCCGT247900.063.7499547
GTATGCC250050.063.4233845
CGTATGC256650.061.9805844
TGCCGTC260800.060.58423648
TCGTATG267500.059.36342243
CTCGTAT261450.057.95293442
GCCGTCT273700.057.6279549
AGTCACC284700.056.5233228
CAGATCA281350.055.87734234
TCACCAG294450.054.59024430
ACCAGAT293350.054.53549232
TCTCGTA276550.054.20231641
GTCACCA296450.054.14260529
CAGTCAC305100.053.05047227
AAAAAGG288900.053.0203869
CACCAGA302250.052.86161831
CCAGTCA322050.050.16279226
CATCTCG296000.050.1265939
CCAGATC318500.049.78329533
Produced by FastQC (version 0.11.2)