FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8543487
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1430361.6742110100945902TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG847880.9924285013835685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG183900.065.5831946
ATGCCGT187050.064.27511647
GTATGCC189800.063.61721845
CGTATGC193000.062.794844
TGCCGTC196850.060.9700848
TCGTATG203250.059.5601343
GTCACAC205400.058.6254729
CACACAG205700.058.57042731
CACAGTG206500.058.4062533
ACACAGT206600.058.34799632
CTCGTAT194800.058.19443542
GCCGTCT207600.057.69658749
TCACACA216050.055.79618530
AGTCACA217500.055.7609228
TCTCGTA202100.054.72680341
CAGTCAC221800.054.66401327
AAAAAGG221700.052.79874469
ACAGTGA226000.052.69479834
GATCTCG208400.052.37589339
AGTGATC208450.052.28180736
Produced by FastQC (version 0.11.2)