FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7728205
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1783242.3074439666132043TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG522510.6761078413422005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG221000.066.3758546
ATGCCGT225500.064.94417647
GTATGCC226200.064.8186345
ACTTAGG227450.064.7383432
CTTAGGC228300.064.20769533
CACTTAG230600.063.97411731
TCACTTA232150.063.66587430
CGTATGC232400.063.163644
TTAGGCA230950.063.06722334
TGCCGTC233050.062.79580348
TCGTATG238350.061.57235743
AGTCACT243900.060.70129428
GCCGTCT241350.060.57908649
CTCGTAT231600.060.2834542
TAGGCAT236550.060.24703635
AGGCATC224800.060.1119436
GTCACTT251250.058.85688829
CAGTCAC256350.058.11739727
TCTCGTA236150.057.71949441
AAAAAGG259600.055.35022469
Produced by FastQC (version 0.11.2)