FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11732208
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1948531.6608382667610393TruSeq Adapter, Index 12 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG995940.8488939166438235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG246250.066.2785946
ATGCCGT250650.064.8943447
GTATGCC254750.064.1751945
CGTATGC266300.061.54723744
TGCCGTC264400.061.5466848
TCGTATG274900.059.69708343
GCCGTCT277200.058.59269749
CTCGTAT265700.057.6222842
GTAATCT268400.056.1938337
TGTAATC270550.055.77277436
GTCACCT296700.055.29654729
AGTCACC299050.055.0708728
TCTCGTA276550.054.83763541
TAATCTC281300.053.68998738
CACCTTG305750.053.52050431
CTTGTAA305700.053.2418934
ACCTTGT313150.052.3088532
AAAAAGG304350.052.02852269
TTGTAAT312500.051.76320635
TCACCTT318350.051.52120630
Produced by FastQC (version 0.11.2)