FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673123
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4780984.47945741841446TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG834470.781842390460599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG585500.067.58498446
ATGCCGT589500.066.9203147
GTATGCC593350.066.71284545
CGTATGC602200.065.904344
TGCCGTC600650.065.5804148
GCTACAT594850.065.2861135
TCGTATG609050.065.1857243
GGCTACA604450.064.9683734
CTACATC582800.064.8127636
GCCGTCT606150.064.78046449
CTCGTAT597300.064.6082442
CGGCTAC615700.064.3920233
ACGGCTA622650.063.93548632
TACATCT593600.063.71491637
TCTCGTA601750.063.42527841
CACGGCT634150.062.77119431
TCACGGC644450.061.85895530
AAAAAGG616600.061.7870269
ACATCTC618750.061.2410738
CATCTCG624150.060.68911739
Produced by FastQC (version 0.11.2)