FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9889730
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2476022.503627500447434TruSeq Adapter, Index 10 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611390.6182069682387689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG297950.066.3463746
ATGCCGT302700.065.2020247
GTATGCC306000.064.5552245
CGTATGC312850.063.3181944
TGCCGTC313200.063.01612548
TATCTCG304250.061.80771639
TAGCTTA319500.061.67637634
TCGTATG321700.061.4583443
GCCGTCT322150.061.10477449
CTCGTAT313750.060.60758242
CTTATCT314300.059.7441637
ACTAGCT331500.059.73875832
TTATCTC318000.059.1569138
AGCTTAT330650.059.12704535
CTAGCTT335200.058.90080633
AAAAAGG328350.058.4908569
CACTAGC341100.058.37130731
TCTCGTA323300.058.2944241
TCACTAG348400.057.22747430
GCTTATC330850.056.98499736
Produced by FastQC (version 0.11.2)