FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12994442
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5265574.052170920459686TruSeq Adapter, Index 9 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG925920.7125507967175505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG183330.141083395500938TruSeq Adapter, Index 9 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG650200.067.4740246
ATGCCGT656200.066.80475647
GTATGCC659550.066.5951845
CGTATGC668800.065.787644
TGCCGTC671000.065.33201648
TCGTATG678900.064.829243
GCCGTCT684000.063.96928449
CTCGTAT647500.063.8332842
CAGATCT640650.062.86265637
TCTCGTA651050.062.70099341
AGTCACG710250.062.5720528
CGATCAG717000.061.63039833
CAGTCAC725000.061.41420427
GATCAGA709550.061.3238334
AGATCTC660250.061.142638
GTCACGA725650.061.1052929
AAAAAGG701350.060.86076769
ATCAGAT711300.060.4066635
GATCTCG671150.060.08791739
CCAGTCA741850.059.9597326
Produced by FastQC (version 0.11.2)