FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_sea221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_sea221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11201636
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3864363.4498175087996072TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG792510.7074948694994195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG160390.14318444198686692TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG488850.066.5776946
ATGCCGT494200.065.79323647
GTATGCC496850.065.5811945
CGTATGC505650.064.630944
TGCCGTC504450.064.44298648
TCGTATG514800.063.4218443
GCCGTCT513000.063.20753549
CTCGTAT481250.062.80375342
AGTCACA547750.061.01361528
TCTCGTA485800.060.7949841
GTCACAT549250.060.75741629
ACATCAC541600.060.7313232
CAGTCAC557450.060.08245527
AAAAAGG528100.060.0411969
CATCACG551300.059.51125333
GATCTCG490000.059.4490839
CACATCA556900.059.1627331
TCACGAT531250.059.06799735
ATCACGA548850.058.56926334
TCACATC567250.058.10149830
Produced by FastQC (version 0.11.2)