FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_mrp81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_mrp81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8346473
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2864133.4315452766695587TruSeq Adapter, Index 8 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG476010.5703127536625351No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG141190.16916127327075758TruSeq Adapter, Index 8 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG364600.066.0362946
GTATGCC366200.065.7363845
CGTATGC367850.065.535344
ATGCCGT367750.065.4323447
GTCACAC375700.065.4257529
TCACACT376450.064.9496730
ACTCCAG383550.064.865423
GAACTCC384750.064.783621
AGTCACA382550.064.7330328
TCGTATG374850.064.35750643
CTCGTAT348300.063.95440342
TGCCGTC375350.063.92442748
TCTCGTA344250.063.8650341
ACACGTC393050.063.5980313
CACACGT393250.063.5050412
GCCGTCT376700.063.50302549
CAGTCAC390850.063.3598927
GCACACG396850.062.93765311
CGTCTGA396700.062.89037316
CTGAACT397650.062.78764319
Produced by FastQC (version 0.11.2)