FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_mrp80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_mrp80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6275002
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2725774.343855189209502TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG579060.922804486755542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG318000.067.38132546
GTATGCC320000.067.00223545
CGTATGC321200.066.88080644
TATCTCG313750.066.8520339
TCACGCC320800.066.66733630
ATGCCGT321150.066.5694947
GTCACGC325450.066.00471529
ACTCCAG330400.065.8857323
GAACTCC332450.065.65275621
TCGTATG327700.065.6489643
CTCGTAT320650.065.510842
AGTCACG330650.065.42789528
TGCCGTC326900.065.28348
CACACGT335150.065.2812812
ACGCCAA328150.065.27872532
ACACGTC335300.065.231513
CAGTCAC333850.065.1004827
CACGCCA329200.065.039631
TCTCGTA323300.064.9418141
GCACACG339200.064.5425111
Produced by FastQC (version 0.11.2)