FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_mrp79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_mrp79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7354589
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1270871.7279959491958015TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG527080.7166681917915468No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG94220.12811048992676546TruSeq Adapter, Index 4 (98% over 63bp)
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGGCCTCTTCCAGCCATAAGACCCCAT77530.10541717558928174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG164400.065.3261246
CGTATGC168200.064.0339944
GTATGCC168350.063.9158945
ATGCCGT169300.063.55637447
ACTCCAG178400.061.79586423
TCGTATG175650.061.5733943
AGTCACT178100.061.5095528
GAACTCC179900.061.4311321
TGCCGTC175700.061.24170348
CTCGTAT159800.061.13883642
ACACGTC182250.060.8932913
CACACGT182500.060.82960512
GCACACG184200.060.2869311
CAGTCAC184300.059.72154227
TCTCGTA161650.059.58547241
ACTGACC180250.059.3549232
GAGCACA189400.058.7956779
ACGTCTG189650.058.44452315
CTGAACT189850.058.35017819
CACGTCT190500.058.29239714
Produced by FastQC (version 0.11.2)