FastQCFastQC Report
Sat 24 Dec 2016
H2GKGBGX2_n01_mrp78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GKGBGX2_n01_mrp78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532585
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3374354.479670657549832TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600250.7968711936207823No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGGCCTCTTCCAGCCATAAGACCCCAT89520.11884366389493116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG404600.067.3347946
TATCTCG389000.067.0898539
GTATGCC407650.066.9067345
ATGCCGT406800.066.8773647
CGTATGC409300.066.7629944
CCGATGT412800.066.163533
ACTCCAG418550.066.08887523
TCGTATG414400.066.0578943
CTCGTAT403400.065.704142
GAACTCC422300.065.6868821
ATGTATC399250.065.5229836
CGATGTA416350.065.4916434
CACACGT426700.065.4171412
ACACGTC427400.065.35712413
TGCCGTC417800.065.11658548
CAGTCAC425050.064.9880727
GCACACG429850.064.98546611
TCTCGTA403900.064.8796841
CACGTCT430450.064.7092614
CTGAACT430050.064.691819
Produced by FastQC (version 0.11.2)