FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30523822
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC4197071.3750145705868682TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC465550.042.3284344
TCGTATG475750.041.2911643
CTCGTAT472350.041.2247442
TCTCGTA470050.041.15465541
TATCTCG470850.041.01363439
CGTCTGA493500.040.89763616
ACGTCTG497300.040.62118515
ACACGTC498900.040.60984413
ATCTCGT476950.040.57712640
CACGTCT509750.039.72820314
GCTTATC493950.039.1303336
CACACGT522000.038.90080312
GCACACG524200.038.75795411
CGGAAGA536050.038.0377854
CACTAGC525550.037.92852831
ACTAGCT524050.037.90701732
ATCGGAA539900.037.8611952
GTCACTA528650.037.70207629
TCGGAAG541500.037.6915743
TAGCTTA523900.037.6321534
Produced by FastQC (version 0.11.2)