FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44013947
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC6870011.560871148411207TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC774950.041.8065944
AATCTCG757650.041.374339
CGTCTGA818400.041.164516
TCTCGTA769800.040.9565541
CTCGTAT774200.040.94845642
ACGTCTG825100.040.8467915
TCGTATG791100.040.8275643
GATCGGA822350.040.8040731
ATCGGAA837700.040.7125242
ACACGTC830800.040.6961213
ATCTCGT780650.040.2714440
TCGGAAG847150.040.2177243
CACGTCT842350.040.109714
CGGAAGA854200.039.919054
GCACACG855550.039.59733211
CACACGT863450.039.2333412
GTCACAC883250.037.68673329
TTGAATC840850.037.29762636
GAACTCC901650.037.28830721
GAATCTC847650.037.09763338
Produced by FastQC (version 0.11.2)