FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39525837
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2997540.7583748321382796TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC346300.041.78979544
CTCGTAT349050.040.8739742
TCGTATG353600.040.86462843
TCTCGTA345150.040.7684841
ATCTCGT356250.039.41753840
CATCTCG355800.039.29391539
CGTCTGA375650.039.23856416
ACACGTC384600.038.56628413
ACGTCTG382650.038.56207715
CACGTCT395900.037.41591614
GCACACG409750.036.29026411
CACACGT416050.035.67742512
ATCGGAA422400.035.5754972
CGGAAGA423250.035.4454774
TCGGAAG425650.035.2560463
GATCGGA420850.035.0596961
CTTAGGC412450.035.0426233
TTAGGCA420300.034.30961234
ACTTAGG433750.033.6768632
TAGGCAT422650.033.66622535
Produced by FastQC (version 0.11.2)