FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38938758
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC9423942.4201953231276665TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1070600.042.8600544
CGATCTC1038700.041.84226638
CTCGTAT1074100.041.8042342
CACGATC1038400.041.80318536
TCTCGTA1058250.041.78994441
GATCTCG1041950.041.78656439
TCGTATG1095700.041.76149743
ACGATCT1038800.041.7554337
CGTCTGA1120700.041.75445616
ACACGTC1126850.041.69137613
TCACGAT1062400.041.60635435
ACGTCTG1126200.041.59252515
GATCGGA1123650.041.5275421
ATCACGA1081450.041.5180334
CATCACG1101700.041.47780233
ATCGGAA1143700.041.4732932
ATCTCGT1053700.041.35003740
CACGTCT1139550.041.1747114
TCGGAAG1150200.041.1726153
CGGAAGA1152200.041.0495384
Produced by FastQC (version 0.11.2)