FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33418172
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC17773735.31858235692844TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG506180.1514684884619063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1999450.042.59212544
CTCGTAT1977750.042.2769342
AATCTCG1962100.042.2537139
TCTCGTA1968300.042.25294541
TCGTATG2012400.042.2423543
ATCGGAA2059750.042.0885662
GATCGGA2026750.042.0872271
ATCTCGT1974400.042.0286840
CGTCTGA2063050.041.83411416
GTAATCT1980350.041.7622937
TCGGAAG2074150.041.7541163
ACGTCTG2069400.041.72337715
ACACGTC2075750.041.69661713
TGTAATC2000800.041.38687536
CACGTCT2089950.041.36643614
CGGAAGA2098500.041.2538344
TAATCTC2008550.041.23418438
CACACGT2099800.041.2257612
TTGTAAT2037400.041.19515235
GCACACG2104450.041.14875411
Produced by FastQC (version 0.11.2)