FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26857519
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1051140.39137643354175794TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG847910.3157067486389938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC130450.039.73398244
TCGTATG131450.039.28107543
CTCGTAT130350.039.1059342
TCTCGTA129850.038.8496741
CATCTCG148300.034.045139
ATCTCGT151850.033.45244640
CGTCTGA178150.029.576116
ACGTCTG180800.029.17964615
GTCACCA180500.028.97067529
AGTCACC180300.028.88095928
GATCATC176800.028.5813136
AGATCAT180400.028.46214935
CCAGATC185150.028.15964733
ATCGGAA189200.027.7347352
ACACGTC191600.027.68397913
CAGATCA187700.027.65988234
GATCGGA188150.027.4479181
CAGTCAC190600.027.4473527
TCGGAAG198800.026.4943
CGGAAGA201200.026.1779694
Produced by FastQC (version 0.11.2)