FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29530124
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8155722.761830597121773TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG767000.25973477117806887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC886350.042.8591144
TCGTATG892150.042.49895543
CTCGTAT888950.042.43866742
TCTCGTA890200.042.3666541
ATCTCGT898150.041.9861740
CGCCAAT899700.041.9734833
TATCTCG898450.041.9645239
ACGCCAA906150.041.73554232
GTCACGC917000.041.4098729
GCCAATA919300.041.02114534
CACGCCA923450.040.9962731
AGTCACG930700.040.8950728
TCACGCC926100.040.88140530
CAATATC928850.040.50485636
CCAATAT934600.040.42748335
ATATCTC953600.039.55562238
CAGTCAC981450.038.9642227
CCAGTCA981450.038.9059426
TCCAGTC992000.038.5607925
AATATCT990600.038.14225837