Basic Statistics
Measure | Value |
---|---|
Filename | H2GJMBGX2_n01_mb12.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27529051 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 1284489 | 4.665939991901646 | TruSeq Adapter, Index 4 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 89096 | 0.32364355749132073 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 145015 | 0.0 | 42.51835 | 44 |
CTCGTAT | 142435 | 0.0 | 42.1761 | 42 |
TCGTATG | 145930 | 0.0 | 42.15376 | 43 |
TCTCGTA | 141510 | 0.0 | 42.153214 | 41 |
ATCTCGT | 141425 | 0.0 | 41.9789 | 40 |
AATCTCG | 141290 | 0.0 | 41.91275 | 39 |
ACCAATC | 140920 | 0.0 | 41.579784 | 36 |
CCAATCT | 142335 | 0.0 | 41.4916 | 37 |
CAATCTC | 142680 | 0.0 | 41.411316 | 38 |
GACCAAT | 144790 | 0.0 | 41.34966 | 35 |
ACTGACC | 147635 | 0.0 | 41.244232 | 32 |
CTGACCA | 148605 | 0.0 | 40.905365 | 33 |
TGACCAA | 148025 | 0.0 | 40.77428 | 34 |
GTCACTG | 152500 | 0.0 | 40.5042 | 29 |
AGTCACT | 152885 | 0.0 | 40.503147 | 28 |
CAGTCAC | 153430 | 0.0 | 40.41814 | 27 |
CCAGTCA | 153395 | 0.0 | 40.397167 | 26 |
CGTCTGA | 154555 | 0.0 | 40.37329 | 16 |
CACTGAC | 151695 | 0.0 | 40.341797 | 31 |
ACGTCTG | 155120 | 0.0 | 40.247948 | 15 |