FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27529051
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC12844894.665939991901646TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG890960.32364355749132073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1450150.042.5183544
CTCGTAT1424350.042.176142
TCGTATG1459300.042.1537643
TCTCGTA1415100.042.15321441
ATCTCGT1414250.041.978940
AATCTCG1412900.041.9127539
ACCAATC1409200.041.57978436
CCAATCT1423350.041.491637
CAATCTC1426800.041.41131638
GACCAAT1447900.041.3496635
ACTGACC1476350.041.24423232
CTGACCA1486050.040.90536533
TGACCAA1480250.040.7742834
GTCACTG1525000.040.504229
AGTCACT1528850.040.50314728
CAGTCAC1534300.040.4181427
CCAGTCA1533950.040.39716726
CGTCTGA1545550.040.3732916
CACTGAC1516950.040.34179731
ACGTCTG1551200.040.24794815