FastQCFastQC Report
Tue 14 Feb 2017
H2GJMBGX2_n01_mb11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2GJMBGX2_n01_mb11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41060050
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3191850.7773614498764615TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC361800.041.8725944
CGATGTA358800.041.8031434
TCTCGTA355300.041.78340541
TCGTATG362100.041.70387343
CTCGTAT359350.041.66777842
ACCGATG362700.041.44462632
CACCGAT363550.041.4141931
CCGATGT364250.041.2078733
TATCTCG360500.041.1126739
ATCTCGT365800.040.63757340
CGTCTGA375800.040.63386516
GTCACCG373550.040.38807329
ACACGTC380400.040.21243713
GATCGGA375050.040.193481
TCACCGA375950.040.16525330
ACGTCTG381050.040.1212915
ATCGGAA385150.039.913342
CACGTCT393450.038.90136314
TCGGAAG396500.038.7359543
CGGAAGA400050.038.3646744
Produced by FastQC (version 0.11.2)