FastQCFastQC Report
Wed 10 Apr 2024
H2G7GDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G7GDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017675221
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAACTGT51353661750.461739305726894No Hit
ATTGACAGTC45319434744.53231617005245No Hit
AGATCTCGGT181078321.7793330943251886Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG54914040.5396028012359357No Hit
NACGAACTGT17778630.17469846600500064No Hit
NTTGACAGTC15672400.15400198095223153No Hit
ACGTACTGTG13350000.13118134081010507No Hit
AACGAACTGG12720810.12499871999929534No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)