FastQCFastQC Report
Thu 7 Mar 2024
H2G55DRX5_n01_AtbZIP72_AmpPoolHm-B_ah.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G55DRX5_n01_AtbZIP72_AmpPoolHm-B_ah.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064267
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT121750.29956201204300803TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG54940.13517812683074218TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT46190.113649029455988TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)