FastQCFastQC Report
Thu 7 Mar 2024
H2G55DRX5_n01_AtbZIP48_AmpPoolHm-B_ah.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G55DRX5_n01_AtbZIP48_AmpPoolHm-B_ah.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101952
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT11471.1250392341494035TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTAT5410.5306418706842435TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG4970.48748430634023854TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC3250.31877746390458256TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTATG2960.29033270558694285TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTATGCC2010.19715160075329569TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)