FastQCFastQC Report
Thu 7 Mar 2024
H2G55DRX5_n01_AtbZIP43_AmpPoolHm-B_ah.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G55DRX5_n01_AtbZIP43_AmpPoolHm-B_ah.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143510
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT14921.0396488049613266TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT7060.4919517803637377TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6900.48080273151696745TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATG3380.23552365688802174TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC2410.16793254825447704TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)