FastQCFastQC Report
Wed 13 Sep 2017
H2G3VBGX3_n02_rice2016dsproject_plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G3VBGX3_n02_rice2016dsproject_plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11801200
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGCAGCTAATCGGGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAAC1664401.4103650476222755No Hit
ACTCAGGACTGGGTATCCATGCCAGGTGTTATACCGGTGGCTTCAGGGGG922800.7819543775209301No Hit
ACATAGAATAGATTACTAGATTGAAATAGTAGTCTAATTCAATTTCTTTT690660.5852455682472969No Hit
AGGTTGCACCGACCCTGTTGCCAAGAACTTCGACCCAACGGCGAGGAGCG585920.49649188218147305No Hit
GGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGACTTTAGCCATCT536370.45450462664813746No Hit
GTGCAGTAGATGTACGTACGTGTACCGTATAGATGTACGTATTCGTGTGG486100.4119072636681016No Hit
GTGCAGTACCTCCACCCCAAGGACGGCGTCTACCCGGAGAAGGTCAACGC377280.3196963020709758No Hit
ATACAGAGGAGACTCGATTGATCTATTCGGTTTTGGCAGCTTTGAACGTT352570.2987577534488018No Hit
AGTTAAAACTATACTGCATTAATTAATTTTACCTTGAGAACATACGTGGT346470.293588787580924No Hit
CTGCAGGAGAGTGTGCGTGTGTGTACGCGGTGCAGTAGATGTACGTACGT336500.28514049418703186No Hit
ATATAGAACACTCATATCGATAAAATGGTTTGAACTATTTACTAGAAGGG323980.27453140358607603No Hit
TAGCAGCACAACGCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTT296850.25154221604582583No Hit
AGCCATAGCTGGGCATGACAAGTTTTTGTTAATAATAATATAATATAAAG244480.20716537301291396No Hit
ATGCATGAGTGCCTGTGTGATGGACGATGTAATGATCATGTAAATTGTGC239080.20258956716266144No Hit
AGACAATACCCCACCCCAGGAATAGCTGATTTGAGGATGATCAATTTAGA214330.1816171236823374No Hit
ATGTATAAAAAAGGAGAAAGTTTTTGGATTTGAAGAAAAAAGTAGGAATT208490.17666847439243466No Hit
ACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGA194040.16442395688574044No Hit
GGGTGGAAGAATGTGTTAAATGTACGTGTAAATTATCGTCGAGTTGGGAG193940.1643392197403654No Hit
AGCCATAGCTGGGCATGACAAGTTTTTGTTAATAATAATATAATATAAAA188700.15989899332271293No Hit
AGCTATAACAACATGATCCATATCATCATCAGCAGCAAAGTTCACTTGTG180380.15284886282750906No Hit
CTGCAGAACACGTGCCTCAAGACGTGGGGCGGGCAGCCGGAGAACGTGAA175600.14879842727858184No Hit
ATGTGGAAGCTGCCCATGTTCGGGTGCACCGACGCCACCCAGGTGCTCAA174340.14773073924685626No Hit
AGGTTGAAGAGCATGCATATGTGAATGCTAGCACCAGGCTAATCATATGC172370.14606141748296783No Hit
GAGCAGGAGAACGTCAAGCGTGTGCAGCTGGCTGAGCAGTACTTGAGCGA164950.1397739212961394No Hit
ATGCTGTATTAAGTAATAACAAGAAATATATCTCCTCTACTTTTTCCTGA164590.1394688675727892No Hit
AGGCATATGTGAATGAAATCTATGAAAGCTCCGGCTATATATATTTATAC162390.1376046503745382No Hit
TTGCATAACTTGTGAAACCATGCAATGGAACTTCTAAAAATCCCTCTCCA155410.13168999762735994No Hit
AGGCATATGTGAATGAAATCTATGAAAGCTCCGGCTATATATATTTAAAA152440.12917330440972105No Hit
ACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTT150450.1274870352167576No Hit
GAGCAGTACTTGAGCGAGGCTGCTCTTGGTGACGCTAACTCCGACGCCAT146500.12413991797444326No Hit
ATCGAGAACCTCTTCGACCACGTCGCCGACCCCGTCGCCAACAACGCCTG142400.12066569501406636No Hit
GGACAGACAATACCCCACCCCAGGAATAGCTGATTTGAGGATGATCAATT128650.10901433752499745No Hit
ACGCAGAGTGAATGGGTCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGG127920.10839575636375962No Hit
GGGCAGGACCGAGGGCTACTTCGACAAGTGAATGAATCCACAGTGGCTTT127360.10792122834965935No Hit
AGGCTGCTCTTGGTGACGCTAACTCCGACGCCATGAAGACTGGTTCCTTC127120.10771785920075926No Hit
AGGTAGCGGCGAGACGAGCCGTTTAAATAGGTGTCAAGTGGAAGTGCAGT126110.10686201403247127No Hit
GAGTAGAATTACGAGGATCGATCGATCTACCGAGGTTTCGCCGCCATTGT124950.10587906314612074No Hit
AGCAAGAAGAGGTCCCAGGGCATCCGGTGCGACTACATCGGCTCAGCCAC124360.10537911398840796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGTG374650.042.659544
TAATCGG202700.042.56861511
CTAATCG205000.042.29528410
GGCTTTA195450.042.1270420
TCGTAAC202100.041.87481744
GCAGAGT411350.041.789063
CTCGTAA204300.041.55451243
ACGCAGA403150.041.4934771
AATGGGG171000.041.47960711
ATCGGGT207700.041.47854213
GCTCGTA205500.041.38802342
GCTTTAG200800.041.11435721
GAATGGG349550.041.08754710
ACTCAGG147250.041.02511
CGTAAGC74600.040.993719
CTGGGTA136250.040.966159
GAGTGAA378800.040.9572376
CGCAGAG434750.040.7987372
ATACCGG128300.040.72711631
CGTATAG70850.040.7099326
Produced by FastQC (version 0.11.2)