FastQCFastQC Report
Wed 13 Sep 2017
H2G3VBGX3_n01_rice2016dsproject_plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2G3VBGX3_n01_rice2016dsproject_plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11801200
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCT197350.013.9450021
TCAACCG139000.013.9398321
TCCACAC236000.013.8985031
ACACCTT198150.013.89464952
CCACACT237300.013.88853552
TTCCACC148800.013.8830222
AATCCTT127600.013.852512
GTCCTCT169850.013.8481412
CCTACGT123000.013.8413376
TCGACGC252800.013.8401444
CGACGCT252700.013.8400815
CCTCTCT188600.013.8337254
CTTCGTC196700.013.8333682
TCTCGAC254400.013.8327822
CCTTCGT198550.013.8315195
TCCTCTG170250.013.8280573
TACTGCA192700.013.827261
CAACCGA141250.013.8271772
AACCGAT141000.013.8269853
CTCGACG254200.013.8189983
Produced by FastQC (version 0.11.2)