FastQCFastQC Report
Thu 5 Oct 2017
H2FT3BGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FT3BGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263742003
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14082440.533947563900165No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT2890650.10960142742223733No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG2756160.10450212589005022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC1224650.017.6709253
ATACCGG1289050.017.4688916
TATACCG1306450.017.3963725
GGACCAT821100.016.7274326
TACCGGT1277750.016.3835057
TTATACC1337150.016.3554024
TCAGGGG1240600.016.08337418
GGTATTC1170200.016.0042324
GGTATCA466750.015.6697391
GGGGGTA1266900.015.62405721
TTAGTCC486800.015.18238
TGGACCA1002750.015.1554495
ACCGGTG1382850.015.1429598
GTCATAT1496750.015.1111811
GGCTTCA1312400.015.03773714
TTCAGGG1327000.015.01140917
TAAACCG702750.014.9088264
TGTTATA1615900.014.8348512
TGGCATA1224750.014.63179736
TAGTCCA534200.014.6009849
Produced by FastQC (version 0.11.2)