FastQCFastQC Report
Thu 5 Oct 2017
H2FT3BGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FT3BGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263742003
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACCG5425550.013.9278521
CCTCTCT3600450.013.8890374
TCAGAAC4896900.013.8831282
AACCGAT5446050.013.8817573
GCCGAAC7823250.013.8812975
CCACACT3795300.013.8592732
AACTACC4883900.013.8589096
CTTCGTC5439600.013.8543452
CAACCGA5464400.013.8506492
CTTCAGC4306400.013.8440021
CCGAACA7837050.013.8364186
CGCATCC4133350.013.8363565
AGAACTA4905950.013.835244
TCGCATC4139100.013.8313654
TCAGAGC3818000.013.8239021
TACTGCA4430850.013.8191021
CGGAATA4045750.013.8115411
CTCCTAC2523250.013.8061094
TCTCGAC3066800.013.8047072
TTGAGCT5596000.013.8032432
Produced by FastQC (version 0.11.2)