FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14417940
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAAATAGGATGAATTGAGACGGTATTTTGTAAATACGTAATTATCTTGAATATATTAACTATTTCTTTATTT392020.2718973723014522No Hit
CCTGCGGGAATTATTTGGAAGATCAAAAAAGAGTGGTATTTGCTAGCAACAACATAATGGAGGCAGTCAATCAAT226310.15696417102581922No Hit
CCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTG175960.12204239995450113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170630.11834561664148971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT122800.048.4229133
AGGCGTA128550.048.1621554
GGCGTAA128850.047.8358735
GCGTAAG129100.047.636536
GTATCCT197600.045.5002117
GGCTACA266750.045.34818662
TGGCTAC267100.045.1347461
TAAAGGC133550.045.0072021
GCCTAAG232550.044.837036
GTAGTGT198350.044.80919312
GCTACAC277250.044.46354763
AAAGGCG134750.044.3593142
CCTAAGT228900.043.6774447
CTACACC281200.043.6425664
CTTGTTA354350.043.47517422
GGACTTT266450.043.28762455
CTGCGGG42550.043.2058642
TGTATCC212900.043.0569416
AGCCTAA252550.042.8840525
AAGCCTA252650.042.8670774
Produced by FastQC (version 0.11.2)