FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16621124
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248680.1496168369840692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAGC8700.052.7493251
CCTGGGT10900.052.2326351
TAAGCCT5950.049.851753
AAGCCTA14500.049.7142754
AGCCTAA14850.049.7038655
CCTAAGT14000.048.0349277
GCCTAAG15050.047.6684466
CCTGTGC751.6370905E-1146.006931
CATGGGT18350.045.881571
CCTAGGC2200.045.484121
CATAGCG7900.045.405432
AAAGCCT9500.045.019123
GTAAGCC3250.044.5725482
CCTAGGT5850.044.237431
CCGACGC9500.042.4958721
CCATGGT4800.041.693781
CCTGGTA6350.041.2975581
GTATCCT5100.041.248717
AAAAGCC10300.041.1878742
CCAGGTA6400.040.9749221
Produced by FastQC (version 0.11.2)