FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21002705
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354770.16891633720513619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTA20400.054.779694
AGCCTAA20300.054.539955
ACATAGC11850.054.161241
GCCTAAG20900.052.9742136
TAAGCCT9750.052.355193
CCTGGGT15450.051.8147621
AAAGCCT12050.050.9489943
CCTAAGT20600.050.054917
CCCGTGC900.049.8419271
CCTAGGT6900.049.5085371
CATAGCG10250.048.1192632
AAAAGCC13000.046.6956182
CATAGCT4000.046.5629582
CCTAGGC2150.046.5429081
CATGGGT21800.045.7441251
CCCGTGG1600.045.289061
CCTGGGG6650.045.1431241
GTAAGCC5350.045.1285132
GTATCCT6400.044.1852817
TCCTTGT12800.043.1075920
Produced by FastQC (version 0.11.2)