FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21353875
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339870.1591608080500612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA17300.050.838485
ACATAGC15900.050.784111
AAGCCTA17400.049.753664
TAAGCCT8950.049.7126853
CCTGGGT12450.047.6725271
CCTAAGT18000.046.7464567
GCCTAAG18450.046.547946
CCTGTAC900.046.0095371
CCTGTGT3550.045.685521
CATAGCG12900.045.4532432
CCTAGGT8100.044.3054771
AAAGCCT10000.043.4581343
CCTGGGA5400.043.4534451
CCTGGTG9100.043.2287371
CTTGGGT24750.041.5481
CCCGTGG2500.041.408581
CCTAGGC1850.041.035531
CATGGGT22150.040.972371
GTATCCT5900.040.9146117
CCCAGGC2800.040.669141
Produced by FastQC (version 0.11.2)