FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17105002
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231800.13551591516914174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCT12750.054.6426053
AGCCTAA24250.053.7616775
GCCTAAG24550.052.9642226
ACATAGC9800.052.4642831
AAGCCTA25400.052.2780954
CCTAAGT25150.051.4199267
CCTGGGT10600.049.1557851
AAAGCCT13050.048.100673
CCCAGGC2650.046.877041
CCTAGGC2000.046.584061
GTATCCT7850.046.56780617
CATAGCG11500.046.4866072
CCCGTAC900.046.0089451
GGCCCTC306.3228916E-445.986752
ACATGGC3850.045.7101861
ATCCTTG7400.045.6709619
CTAAGTA135000.045.3420878
AGACACA43400.045.2514831
AAGACAC42500.045.23295230
AAAAGCC14000.044.590732
Produced by FastQC (version 0.11.2)